Journal:

Article Title: N -formyl-methionyl-leucyl-phenylalanine (fMLP) inhibits tumour necrosis factor-alpha (TNF-?) production on lipopolysaccharide (LPS)-stimulated human neutrophils

doi: 10.1046/j.1365-2249.1998.00631.x

Figure Lengend Snippet: (a) CD14 expression and (b) FITC-LPS binding of fMLP-treated polymorphonuclear neutrophils (PMN). PMN were treated (––) or not (·····) with fMLP (10−6m) for 1 h at 37°C. Then the cells were incubated with FITC-LPS (1 μg/106 PMN) during 1 h at 37°C or with MoAb anti-CD14 (0.4 μg/106 PMN) for 30 min at 4°C followed by goat anti-mouse IgG-FITC. Fluorescence intensity of 10 000 cells was analysed for each sample. The solid thin graphs represent the background fluorescence of cells incubated with (a) an isotype-matched IgG2b control MoAb and (b) buffer. Abscissa, Fluorescence intensity; ordinate, number of cells.

Article Snippet: Lipopolysaccharide from Escherichia coli O111:B4 (LPS), FITC-labelled LPS from E. coli O111:B4 (FITC-LPS), polymixin B, actinomycin D, fMLP, N -t-BOC-Phe-Leu-Phe-Leu-Phe ( N -t-BOC), pertussis toxin from Bordetella pertussis (PT), polyclonal antibodies anti-TNF-α, FITC-conjugated F(ab′) 2 goat anti-mouse IgG (Fc-specific) and FITC-conjugated goat anti-rabbit IgG were obtained from Sigma Chemical Co. (St Louis, MO).

Techniques: Expressing, Binding Assay, Incubation, Fluorescence

Journal:

Article Title: N -formyl-methionyl-leucyl-phenylalanine (fMLP) inhibits tumour necrosis factor-alpha (TNF-?) production on lipopolysaccharide (LPS)-stimulated human neutrophils

doi: 10.1046/j.1365-2249.1998.00631.x

Figure Lengend Snippet: CD11/CD18 expression of fMLP-treated polymorphonuclear neutrophils (PMN). PMN were treated (––) or not (·····) with fMLP (10−6m) for 1 h at 37°C. Then the cells (106) were incubated for 30 min at 4°C with 3 μl of MoAbs FITC–anti-CD18, FITC–anti-CD11a/CD18, PE–anti-CD11b/CD18, or PE–anti-CD11c/CD18. Fluorescence intensity of 10 000 cells was analysed for each sample. Abscissa, Fluorescence intensity: FL-1, FITC; FL-2, PE; ordinate, number of cells.

Article Snippet: Lipopolysaccharide from Escherichia coli O111:B4 (LPS), FITC-labelled LPS from E. coli O111:B4 (FITC-LPS), polymixin B, actinomycin D, fMLP, N -t-BOC-Phe-Leu-Phe-Leu-Phe ( N -t-BOC), pertussis toxin from Bordetella pertussis (PT), polyclonal antibodies anti-TNF-α, FITC-conjugated F(ab′) 2 goat anti-mouse IgG (Fc-specific) and FITC-conjugated goat anti-rabbit IgG were obtained from Sigma Chemical Co. (St Louis, MO).

Techniques: Expressing, Incubation, Fluorescence

Journal:

Article Title: N -formyl-methionyl-leucyl-phenylalanine (fMLP) inhibits tumour necrosis factor-alpha (TNF-?) production on lipopolysaccharide (LPS)-stimulated human neutrophils

doi: 10.1046/j.1365-2249.1998.00631.x

Figure Lengend Snippet: FACS analysis of IFN-γ-treated polymorphonuclear neutrophils (PMN). (a) PMN were treated (·····) or not (––) with IFN-γ (100 U/ml) for 1 h at 37°C. Then the cells were incubated with FITC-LPS (1 μg/106 PMN) during 1 h at 37°C (b) or with MoAb anti-CD14 (0.4 μg/106 PMN) for 30 min at 4°C followed by goat anti-mouse IgG-FITC (a). Fluorescence intensity of 10 000 cells was analysed for each sample. The solid thin graphs represent the background fluorescence of cells incubated with (a) an isotype-matched IgG2b control MoAb and (b) buffer.

Article Snippet: Lipopolysaccharide from Escherichia coli O111:B4 (LPS), FITC-labelled LPS from E. coli O111:B4 (FITC-LPS), polymixin B, actinomycin D, fMLP, N -t-BOC-Phe-Leu-Phe-Leu-Phe ( N -t-BOC), pertussis toxin from Bordetella pertussis (PT), polyclonal antibodies anti-TNF-α, FITC-conjugated F(ab′) 2 goat anti-mouse IgG (Fc-specific) and FITC-conjugated goat anti-rabbit IgG were obtained from Sigma Chemical Co. (St Louis, MO).

Techniques: Incubation, Fluorescence

Journal:

Article Title: N -formyl-methionyl-leucyl-phenylalanine (fMLP) inhibits tumour necrosis factor-alpha (TNF-?) production on lipopolysaccharide (LPS)-stimulated human neutrophils

doi: 10.1046/j.1365-2249.1998.00631.x

Figure Lengend Snippet: Effect of fMLP on the expression of membrane TNF-α. Polymorphonuclear neutrophils (PMN; 3 × 106/ml) were treated with fMLP (10−6 m) during 15 min and then stimulated with LPS (5 μg/ml) for 1 h at 37°C. After washing, the cells (106 PMN) were incubated during 30 min at 4°C with 25 μg/ml of an anti-TNF-α polyclonal antibody, followed by goat anti-rabbit FITC-IgG. The solid thin graph represent the background fluorescence of non-stimulated cells (control). The solid histogram was obtained using, as a control, a normal rabbit IgG. Fluorescence intensity of 10 000 cells was analysed for each sample.

Article Snippet: Lipopolysaccharide from Escherichia coli O111:B4 (LPS), FITC-labelled LPS from E. coli O111:B4 (FITC-LPS), polymixin B, actinomycin D, fMLP, N -t-BOC-Phe-Leu-Phe-Leu-Phe ( N -t-BOC), pertussis toxin from Bordetella pertussis (PT), polyclonal antibodies anti-TNF-α, FITC-conjugated F(ab′) 2 goat anti-mouse IgG (Fc-specific) and FITC-conjugated goat anti-rabbit IgG were obtained from Sigma Chemical Co. (St Louis, MO).

Techniques: Expressing, Incubation, Fluorescence

Journal: Cancers

Article Title: Fibroblasts Promote Resistance to KRAS Silencing in Colorectal Cancer Cells

doi: 10.3390/cancers16142595

Figure Lengend Snippet: List of anti-human antibodies used for flow cytometry.

Article Snippet: CD44 , FITC , DB105 , 130-113-896 , Miltenyi Biotec.

Techniques: Cytometry

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD64 , REA987 , 50 , 130-116-195 , FITC , Miltenyi Biotec.

Techniques: Imaging

Journal: Discover oncology

Article Title: Preclinical efficacy of CBR-5884 against epithelial ovarian cancer cells by targeting the serine synthesis pathway.

doi: 10.1007/s12672-024-01013-0

Figure Lengend Snippet: Fig. 5 CBR-5884-impaired stemness of EOC cells and enhanced chemotherapy sensitivity. A SKOV3 and ID8 cells were treated with CBR- 5884 (50 μM) or dimethyl sulfoxide (DMSO; control) for 5 days, and tumorsphere formation ability was accessed by sphere formation assay. Representative images (left) and quantification data (right) are shown. B Cell cytometry was performed to detect CD44 expression in SKOV3 and ID8 cells after treatment with 30 μM CBR-5884 for 48 h. Representative images (left) and quantification data (right) are shown. C Quanti- tative polymerase chain reaction assay was conducted to measure the mRNA expression levels of stem-related genes in SKOV3 cells, includ- ing ALDH1A1, NANOG, SOX2, EPCAM, and POU5F1 after treatment with 30 μM CBR-5884 for 48 h. D SKOV3 and ID8 cells were treated with DMSO, carboplatin (20 μM), CBR-5884 (30 μM), and carboplatin (20 μM) plus CBR-5884 (30 μM) for 72 h. Cell viability was measured using CCK-8 assay

Article Snippet: To detect CD44-positive cells, a FITC anti-mouse CD44 (IM7; 1:200 dilution, Cat. No. FITC-65117; Proteintech, USA) antibody was used.

Techniques: Control, Tube Formation Assay, Cytometry, Expressing, Polymerase Chain Reaction, CCK-8 Assay